Location: Dairy Forage Research
Project Number: 5090-21000-056-09-I
Project Type: Interagency Reimbursable Agreement
Start Date: Sep 1, 2013
End Date: Aug 31, 2017
The proposed research aims to determine and confirm the genes involved in flowering time and to provide key information on the genetic mechanisms controlling flowering responses of switchgrass. The specific objectives are to: 1. Perform genome-wide association analysis of SNPs with flowering time in both lowland and upland panels. 2. Identify genes for regulation and control of flowering time. 3. Confirm genes associated with flowering time in the segregating populations. 4. Examine expression profiles of candidate genes controlling flowering time at various developmental stages.
We will be using two linkage populations and two association panel populations to identify chromosome regions with significant positive and negative effects on the flowering time of switchgrass. We will dissect genes within these regions to identify SNP markers that can be used for selection and to identify the genes most responsible for genetic variation in flowering time. The project will rely on both field and laboratory evaluations, the latter including deep sequencing, reference-genome alignment, candidate-gene identification, transcriptome sequencing, and expression analysis.