Location: Virus and Prion Research
Project Number: 5030-32000-108-17-S
Project Type: Specific Cooperative Agreement
Start Date: Sep 15, 2013
End Date: Sep 30, 2014
To develop a collaborative program between the United States and China to monitor clinical disease consequences and evolution of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) strains in Asia and the United States to provide core knowledge into both single and dual infections with these two economically important viruses.
Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences (CAAS), will sample porcine reproductive and respiratory syndrome virus (PRRSV) and porcine epidemic diarrhea virus (PEDV) field isolates and gather information on clinical disease severity associated with PEDV, PRRSV, or cases of dual infection with these agents. Scientists in Harbin will obtain associated nucleotide sequences for select regions of the viral genomes and share the obtained sequences with USDA-ARS scientists at the National Animal Disease Center (NADC). NADC scientists will communicate and coordinate with HVRI scientists as to what field isolates are sampled (perceived severity of the disease, other pathogens present, wide geographic area and time of sampling) and regions of the viral genomes that are targeted. NADC scientists will travel to HVRI in order to troubleshoot potential problems in nucleotide sequence derivation and associated clinical disease analysis. HVRI scientist(s) will visit NADC for training. Obtained nucleotide sequences will be transmitted to NADC through electronic mail. NADC will align the sequences to other viral strains to understand whether there are additional genetically distinct PEDV and PRRSV isolates causing increased disease symptoms of which both the United States and China should be cognizant of and take needed steps to prevent viral transmission.