Location: Northwest Irrigation and Soils Research
Project Number: 2054-21220-004-00-D
Project Type: In-House Appropriated
Start Date: Jun 2, 2013
End Date: Jun 1, 2018
1. Identify molecular markers and their genetic map positions for priority sugar beet traits, including host plant resistance to curly top, root rots, and abiotic stresses. [NP301, C1, PS1B] 1.1. Whole genome re-sequencing of sugar beet public breeding line KDH13 for genetic variation analysis. 1.2. Identify a large representative set of single nucleotide polymorphism (SNP) markers for genotyping of mapping populations and germplasm. 1.3. Construct high density genetic linkage map to identify DNA markers closely linked to genes regulating resistance to curly top. 2. Improve germplasm screening procedures for host plant resistance, and incorporate disease management options into production practices through enhanced understanding of plant pathogen etiology and interactions with host resistance, pests, and abiotic stresses. [NP303, C3, PS3A] 2.1. Investigate curly top species variation and/or the presence of new curly top species in sugar beet. 2.2. Refine management strategies for curly top and pest control in sugar beet. 2.3. Establish the etiology and management options for an Athelia-like fungus associated with stored sugar beet roots. 2.4. Characterize and exploit the interaction of Rhizoctonia solani and Leuconostoc mesenteroides in sugar beet root rot to improve disease management options. 2.5. Determine the effect of rhizomania in the field on freeze damage to sugar beet roots in storage. 3. Identify novel sources of host plant resistance to diseases (curly top, rhizomania, and root rots), storage, and abiotic stresses (drought and frost), and incorporate them into adapted germplasm. [NP301, C1, PS1A]
Establish a research program to coordinate, interact and collaborate with university scientists to provide sustainable integrated disease and pest management strategies for sugar beet. New sugar beet germplasm with enhanced disease resistance and agronomic qualities will be developed, along with innovative and improved disease management strategies. Specific focus has been placed on alleviating crop losses due to curly top, rhizomania, root rots, spoilage during storage, and seedling frost injury. Genetic research will identify molecular markers associated with curly top resistance and establish their genetic map position. Understanding the etiology of pathogens associated with curly top, rhizomania, root rots, and storage losses, i.e., fungal decay, will lead to improved disease management options and screening methods. Using genetic methods with germplasm from the disease screening nurseries (curly top, rhizomania, Rhizoctonia-bacterial root rot, and storage), we will identify novel genes regulating traits of interest ultimately leading to public release of improved germplasm. New germplasm will be used by the sugar beet industry to enhance disease resistance and improve yields in commercial cultivars. The new genetic and pathogen knowledge generated will also allow our stakeholders to reduce losses by improved disease and postharvest storage management.