Project Number: 5030-32000-117-03-T
Project Type: Trust Fund Cooperative Agreement
Start Date: May 30, 2013
End Date: May 30, 2017
Evaluate bovine coronavirus (BoCV) field isolates using in vitro assays based on infection of cultured macrophages.
Develop in vitro assay using cultured macrophages to compare BoCV strains. Macrophages,isolated from blood of non infected donor animals (bovine), will be cultured in the presence of different BoCV strains. Viral titer will be monitored by VI and PCR and pro-inflammatory cytokines will be monitored using real-time PCR a multiplex ELISA assay.