Project Number: 8072-41420-019-03-A
Project Type: General Cooperative Agreement
Start Date: Feb 20, 2014
End Date: Dec 31, 2017
The overall goal of the project is to determine the prevalence, pathogenic potential, and characterization of E. coli O157:H7 in retail ground veal in four states of the Mid-Atlantic region of the United States. The specific objectives are to: 1) determine the prevalence of E. coli O157:H7 in retail ground veal collected from Maryland, Delaware, Pennsylvania, and Virginia; 2) determine the presence of virulence genes (stx1, stx2, eae, ehxA, and others to be determined) in E. coli O157:H7 isolates; 3) Characterize E. coli O157:H7 isolates by genotypic (pulsed-field gel electrophoresis) methods; and 4) Share meat samples, enrichment broths, and isolates with STEC CAP collaborators for further testing for USDA-FSIS “big six” adulterant serogroups of non-O157 STEC. 5) Collect approximately 200 additional samples which are to be sent to ARS.
We are planning to analyze a total of 1,000 raw ground veal samples (250 from each state, at least 1 kg per sample) collected from retail stores in Maryland, Delaware, Pennsylvania, and Virginia over a period of nine months for detection of E. coli O157:H7 and non-O157 in veal. In addition, isolates recovered from positive samples will be tested for virulence genes and PFGE profiles.