Project Number: 6034-21000-014-18-R
Project Type: Reimbursable
Start Date: Apr 1, 2012
End Date: Mar 31, 2015
To transform citrus with genes encoding selected scFv and evaluate the transgenic citrus for resistance to huanglongbing (HLB).
ARS USHRL Fort Pierce will use these constructs to transform citrus, identify transformed citrus, produce and propagate transformed trees, and screen them for resistance to 'Ca. Liberibacter asiaticus'. ARS U.S. Hortictural Research Laboratory, Ft. Pierce has developed efficient methods for large scale transformation, regeneration and screening of citrus for resistance to Huanglongbing (HLB). ARS Beltsville has developed an array of scFv that bind to critical epitopes on the pathogen which causes huanglongbing in citrus.