Project Number: 5030-32000-105-05-R
Project Type: Reimbursable
Start Date: Jun 1, 2013
End Date: Aug 31, 2016
Objective of work to be done at NADC is shotgun sequencing, library building, and initial assembly and annotation of up to 8 bison isolates of Mycoplasma bovis; comparison with cattle isolates to facilitate rational design of vaccines and diagnostics.
Shotgun sequencing will be completed via pyrosequencing and long-read sequencing chemistry reagents. Draft assemblies will be created using the Newbler assembly or by MIRA (Mimicking Intelligent Read Assembly), as warranted. Annotation approaches include RAST, Glimmer3 and the Artemis genome viewer with BLAST searches.