Location: Corn Insects and Crop Genetics Research
Project Number: 5030-21000-060-00-D
Project Type: Appropriated
Start Date: May 13, 2013
End Date: May 12, 2018
Objective 1: Discover and functionally characterize host genes and pathways that respond to pathogen infection in barley and maize. Sub-Objective 1A: Identification of barley Blufensin1 (Bln1)-mediated response pathways. Sub-Objective 1B: Functional confirmation via integrated reverse genetic analysis. Sub-Objective 1C: Identify and functionally characterize mechanisms of defense against leaf blights with hemi-biotrophic and necrotrophic lifestyles in maize. Objective 2: Identify and analyze the role of pathogen effectors that influence host response in barley and maize. Sub-Objective 2A: Isolation of Blumeria graminis f. sp. hordei (Bgh) effectors to identify plant targets that promote or suppress defense in host and nonhost interactions. Sub-Objective 2B: Identification of candidate effectors produced by S. turcica and C. heterostrophus during interactions with maize.
Large scale sequencing of pathogen genomes, as well as their plant hosts, has provided unprecedented access to the genes and gene networks that underlie host-pathogen interactions. Regulatory focal points critical to these interactions will be investigated. Determination of these focal points will enable the molecular dissection of important disease resistance pathways, as well as the creation of a molecular toolbox from which to apply modern plant breeding methodologies.