Project Number: 2034-22000-010-25-S
Project Type: Specific Cooperative Agreement
Start Date: Jul 1, 2013
End Date: Jun 30, 2016
1) Identify all electrical penetration graph (EPG) waveforms for adult lygus bugs feeding on susceptible cotton squares, and 2) Define the biological meanings of waveforms by correlating them with stylet tip locations and stylet activities, especially ingestion from a certain cell type.
A Lygus hesperus colony will be established in the laboratory. Thereafter, three studies will be performed, to 1) identify L. hesperus EPG waveforms, 2) begin to correlate each waveform with stylet locations in cotton square tissue via cutting stylets and using histological examination to identify cell types penetrated, and 3) begin to correlate waveforms with stylet activities via simultaneous video-recording and EPG-recording of stylet movements in artificial diet.