Location: Crop Improvement and Protection Research2013 Annual Report
1a. Objectives (from AD-416):
The objective of the project is to investigate the relationship between a sugar beet virus and its fungal vector (Polymyxa betae) on viral transmission to sugar beet.
1b. Approach (from AD-416):
Several different research approaches will be followed to attain the objectives of this project. • Real time PCR techniques for the detection and quantification of P. betae in host tissue will be developed • Real time PCR techniques for the detection and quantification of Beet Necrotic Yellow Vein Virus (BNYVV) in host tissue will be developed • The relationship between the extent of fungal colonization of roots and virus titer will be determined using the above noted real time PCR assays. Different combinations of strains of P. betae and of BNYVV will be evaluated. • The ability of the P. betae real time PCR assay to quantify the level of the pathogen in the soil will be evaluated.
3. Progress Report:
This project, begun in April 2013, relates to sub-objective 2.A in the parent project. Determine the effect of variation among Polymyxa betae isolates on prevalence and dominance of soil-borne viruses affecting sugarbeet. Molecular probes were developed through this new project to quantitatively measure levels of three soil-borne viruses affecting Amercian sugarbeet production, Beet necrotic yellow vein virus (BNYVV), Beet soil-borne mosaic virus (BSBMV), and Beet soil-borne virus (BSBV). We confirmed their reliable and efficient amplification, as well as differentiation of each of the three soil-borne viruses targeted in single infections. These are robust and useful probes for quantification of diverse field isolates. The probes will be used for quantification of BNYVV levels. Development of molecular probes for quantification of the Polymyxa betae, the soil-borne organism responsible for transmission of these viruses, is in progress.