Project Number: 2092-22430-002-03-T
Project Type: Trust
Start Date: Apr 1, 2013
End Date: Mar 31, 2016
The objective of this project is to determine which stress response proteins or detoxification enzymes are expressed in response to codling moth exposure to “reduced-risk” insecticides and granulosis virus, as well as to evaluate potential compounds that inhibit pheromone production by codling moth females.
We will: 1) clone gene transcripts encoding codling moth heat shock proteins and detoxification enzymes. Quantify expression levels of codling moth heat shock proteins and detoxification enzymes from untreated eggs, neonates, and adults or those exposed to heat, cold, or sub-lethal doses of Altacor, Delegate, Calypso and granulosis virus; and 2) Clone gene transcripts encoding for codling moth PBAN receptors (PBANR). Express cloned receptors in mammalian cell lines. Verify PBANR activity and determine if PBAN antagonists block receptor activity. Test compounds on female codling moth to determine biological activity in pheromone biosynthesis inhibition.