Location: Genomics and Bioinformatics Research
Project Number: 6066-21310-004-20-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jan 1, 2013
End Date: Dec 31, 2013
The diploid reference sequence has fundamentally changed cotton research. Application of this reference sequence to improve our understanding of the polyploid, cotton genome has been rapid. These basic advances will further our progress of improving the production of U.S. domestic cotton by 1) allowing us to associate traits to one of two duplicated genomes and enabling their selection during breeding and 2) understand the origin of and frequency of introgressed alleles within the Gossypium (G.) hirsutum genome.
Proposed is a significant re-sequencing project by sequencing many accessions of Gossypium (G.) barbadense at relatively low coverage (5x). The justification of re-sequencing G. barbadense instead of G. hirsutum is found in sequence capture data. When examining a hirsutum diversity panel, occasionally rare alleles are found - even in accessions that should not be that diverse. These 'rare' alleles could be introgressions from G. barbadense and while rare in the hirsutum germplasm, they really are not rare alleles within cotton germplasm as a whole. Thus, a solid database of G. barbadense sequence will allow hirsutum to researchers make a value judgment regarding the 'rare' alleles introgressed from G. barbadense. Such a judgment cannot happen without some G. barbadense sequence. There is ample evidence and a history of introgression from G. barbadense in SeaLand cottons and the PeeDee germplasm collections to justify this approach.