Skip to main content
ARS Home » Research » Research Project #424585

Research Project: Innovations that Improve the Efficiency and Effectiveness of Managing and Preserving Ex Situ Plant Germplasm Collections

Location:

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2019. Implementation of citrus shoot tip cryopreservation in the USDA-ARS national plant germplasm system. Acta Horticulturae. 1234:329-334. https://doi.org/10.17660/ActaHortic.2019.1234.43.

Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants Reprint Icon - (Peer Reviewed Journal)
Bettoni, J.C., Bonnart, R.M., Shepherd, A.N., Kretzschmar, A., Volk, G.M. 2019. Successful cryopreservation of Vitis vinifera cv. ‘Chardonnay’ from both in vitro and growth chamber source plants. Acta Horticulturae. 1234:211-218. https://doi.org/10.17660/ActaHortic.2019.1234.28.

Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Leado, A., Ayala Silva, T. 2019. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Acta Horticulturae. 1234:335-342. https://doi.org/10.17660/ActaHortic.2019.1234.44.

Biases induced by using geography and environment to guide ex situ conservation Reprint Icon - (Peer Reviewed Journal)
Reeves, P.A., Richards, C.M. 2018. Biases induced by using geography and environment to guide ex situ conservation. Conservation Genetics. 19:1281-1293. https://doi.org/10.1007/s10592-018-1098-z.

Genetic diversity and domestication history in Pyrus - (Book / Chapter)
Volk, G.M., Cornille, A. 2019. Genetic diversity and domestication history in Pyrus. In Korban SS (ed.) "The Pear Genome". Springer. Switzerland. p. 51-62.

Outcomes of an international workshop to develop training/educational programs for Plant Genetic Resources Management - (Abstract Only)
Volk G, Byrne P, Bretting P. 2018. Outcomes of an international workshop to develop training/educational programs for plant genetic resources management. American Soc. Horticultural Sci. Conference, July 31-Aug 3, 2018. https://ashs.confex.com/ashs/2018/meetingapp.cgi/Paper/28081cgi/Paper/28081.

Chloroplast sequence data differentiate Maleae, and specifically Pyrus, species in the USDA-ARS National Plant Germplasm System Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Henk, A.D., Richards, C.M., Bassil, N.V., Postman, J.D. 2018. Chloroplast sequence data differentiate Maleae, and specifically Pyrus, species in the USDA-ARS National Plant Germplasm System. Genetic Resources and Crop Evolution. 66(1):5-15. https://doi.org/10.1007/s10722-018-0691-9.

Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species - (Peer Reviewed Journal)
Volk, G.M., Shepherd, A.N., Bonnart, R.M. 2018. Successful cryopreservation of Vitis shoot tips: Novel pre-treatment combinations applied to nine species. CryoLetters. 39(5):322-330.

Exploring the fate of mRNA in aging seeds: protection, destruction, or slow decay? Reprint Icon - (Peer Reviewed Journal)
Fleming, M.B., Patterson, E., Reeves, P.A., Richards, C.M., Gaines, T., Walters, C.T. 2018. Exploring the fate of mRNA in aging seeds: protection, destruction, or slow decay? Journal of Experimental Botany. 69(18):4309-4321. https://doi.org/10.1093/jxb/ery215.

Identification of unknown apple cultivars demonstrates the impact of local breeding program on cultivar diversity - (Peer Reviewed Journal)
Gross, B.L., Martinez, M., Wedger, M.J., Volk, G.M., Hale, C. 2018. Identification of unknown apple cultivars demonstrates the impact of local breeding program on cultivar diversity. Genetic Resources and Crop Evolution. 65:1317-1327.

Combining thermotherapy with cryotherapy for efficient eradication of Apple stem grooving virus from infected in vitro-cultured apple shoots - (Peer Reviewed Journal)
Lei, Z., Wang, M., Cui, Z., Chen, L., Volk, G.M., Wang, Q. 2018. Combining thermotherapy with cryotherapy for efficient eradication of Apple stem grooving virus from infected in vitro-cultured apple shoots. Plant Disease. 102: 1574-1580.

Cryotherapy by encapsulation-dehydration is effective for in vitro eradication of latent viruses from ‘Marubakaido’ apple rootstock - (Peer Reviewed Journal)
Bettoni, J.C., Costa, M.D., Souza, J.A., Nickel, O., Volk, G.M., Da Silva, F.N., Kretzschmar, A.A. 2018. Cryotherapy by encapsulation-dehydration is effective for in vitro eradication of latent viruses from ‘Marubakaido’ apple rootstock. Journal of Biotechnology. 269:1-7.

Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System - (Abstract Only)
Volk, G.M., Jenderek, M.M., Walters, C.T., Bonnart, R.M., Shepherd, A.N., Skogerboe, D.M., Hall, B.D., Moreland, B.L., Krueger, R., Polek, M. 2018. Implementation of Citrus Shoot Tip Cryopreservation in the USDA-ARS National Plant Germplasm System. Meeting Abstract. 20.

The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil - (Abstract Only)
Bettoni JC, Bonnart R, Shepherd A, Kretzschmar AA, Volk GM. 2018. The development of a droplet-vitrification method to conserve Vitis collections in the USDA-ARS National Plant Germplasm System and UDESC-CAV Santa Catarina State University in Brazil. Third International Symposium on Plant Cryopreservation. CryoSymp 2018. 26-28 March 2018. Bangkok, Thailand. pg. 24.

Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation - (Abstract Only)
Volk, G.M., Jenderek, M.M., Staats, E.R., Shepherd, A.N., Bonnart, R.M., Ledo, A., Ayala Silva, T. 2018. Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. Meeting Abstract. 57.

Cryopreservation of apple (Malus spp.): development, progress and future prospects Reprint Icon - (Peer Reviewed Journal)
Wang, M., Chen, L., Liu, J., Teixeira Da Silva, J.A., Volk, G.M., Wang, Q. 2018. Cryopreservation of apple (Malus spp.): development, progress and future prospects. Plant Cell Reports. 37(5):689-709. https://doi.org/10.1007/s00299-018-2249-x.

Diversity from genes to ecosystems: A unifying framework to study variation across biological metrics and scales Reprint Icon - (Peer Reviewed Journal)
Gaggiotti, O., Chao, A., Peres-Neto, P., Chiu, C., Edwards, C., Fortin, M., Jost, L., Richards, C.M., Selkoe, K. 2018. Diversity from genes to ecosystems: A unifying framework to study variation across biological metrics and scales. Evolutionary Applications. 11:1176-1193. https://doi.org/10.1111/eva.12593.

Genomic diversity and macroecology of the crop wild relatives of domesticated pea - (Peer Reviewed Journal)
Smykal, P., Hradilová, I., Trnený, O., Brus, J., Rathore, A., Bariotakis, M., Das, R., Bhattacharyya, D., Richards, C.M., Coyne, C.J., Pirintsos, S. 2017. Genomic diversity and macroecology of the crop wild relatives of domesticated pea. Nature Scientific Reports. doi:10.1038/s41498-017-17623-4.

Capturing haplotypes in germplasm core collections Reprint Icon - (Peer Reviewed Journal)
Richards, C.M., Reeves, P.A. 2017. Capturing haplotypes in germplasm core collections. Genetic Resources and Crop Evolution. doi:10.1007/s10722-017-0549-6.

Targeted next-generation sequencing identification of mutations in disease resistance gene anologs (RGAs) in wild and cultivated beets - (Peer Reviewed Journal)
Stevenato, P., Brocenello, C., Pajola, F., Richards, C.M., Panella, L.W., Hassani, M., Formentin, E., Chiodi, C., Concheri, G., Heidari, B. 2017. Targeted next-generation sequencing identification of mutations in disease resistance gene anologs (RGAs) in wild and cultivated beets. Genes. 8(10):264. doi:10.3390/genes8100264.

Germplasm morgue or gold mine? Enhancing the value of plant genetic resource collections for plant breeding - (Abstract Only)
Byrne, P., Volk, G.M., Gardner, C.A., Gore, M., Simon, P.W., Smith, S. 2017. Germplasm morgue or gold mine? Enhancing the value of plant genetic resource collections for plant breeding. National Association of Plant Breeders. Page 1.

Seed biology influences recruitment, demography, restoration success, and population persistence of endangered Dalea carthagenensis var. floridana (Fabaceae) - (Peer Reviewed Journal)
Maschinski, J., Possley, J., Walters, C.T., Hill, L.M., Walters, D., Kreuger, L., Hazelton, D. 2017. Seed biology influences recruitment, demography, restoration success, and population persistence of endangered Dalea carthagenensis var. floridana (Fabaceae). Restoration Ecology. 26: 636-641.

Citrus genebank collections: International collaboration opportunities between the U.S. and Russia Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Samarina, L.K., Kulyan, R., Vyacheslav, G., Malyarovskaya, V., Ryndin, A., Polek, M., Krueger, R., Stover, E.W. 2017. Citrus genebank collections: International collaboration opportunities between the U.S. and Russia. Genetic Resources and Crop Evolution. 65:433-447. https://doi.org/10.1007/s10722-017-0543-z.

Ex situ conservation of germplasm collections from natural populations - (Book / Chapter)

Sampling wild species to conserve genetic diversity - (Book / Chapter)

Cryoprotectants and their components induce plasmolytic responses in sweet potato suspension cells - (Peer Reviewed Journal)
Volk, G.M., Caspersen, A.M. 2017. Cryoprotectants and their components induce plasmolytic responses in sweet potato suspension cells. In Vitro Cellular and Developmental Biology - Plants. 53:363-371.

Temperate tree fruits of North America: Malus Mill., Prunus L., Diospyros L., and Asimina Adans Reprint Icon - (Book / Chapter)
Volk, G.M. 2019. Temperate tree fruits of North America: Malus Mill., Prunus L., Diospyros L., and Asimina Adans. North American Crop Wild Relatives. 2:353-386. https://doi.org/10.1007/978-3-319-97121-6_11.

Ensuring the genetic diversity of apples - (Book / Chapter)
Volk, G.M. 2017. Ensuring the genetic diversity of apples. In: Evans, K.Achieving sustainable cultivation of apples. Burleigh Dodds Science Publishing,Cambridge, UK. p.3-21.

Indaziflam: A new cellulose biosynthesis inhibiting herbicide provides long-term control of invasive winter annual grasses - (Peer Reviewed Journal)
Sebastian, D.J., Fleming, M.B., Patterson, E.L., Sebastian, J.R., Nissen, S.J. 2017. Indaziflam: A new cellulose biosynthesis inhibiting herbicide provides long-term control of invasive winter annual grasses. Pest Management Science. doi:10.1002/ps.4594.

Phylogeography of the wild and cultivated stimulant plant qat (Catha edulis, Celastraceae) in areas of historic cultivation1 - (Peer Reviewed Journal)
Tembrock, L., Simmons, M., Richards, C.M., Reeves, P.A., Reilley, A.A., Curto, M.A., Meimberg, H., Ngugi, G., Demissew, S., Wali Al Khulaidi, A., Mansoor, A., Simpson, S.A., Varisco, D. 2017. Phylogeography of the wild and cultivated stimulant plant qat (Catha edulis, Celastraceae) in areas of historic cultivation1. American Journal of Botany. 104: 538-549.

Decline in RNA integrity of dry-stored soybean seeds correlates with loss of germination potential. - (Peer Reviewed Journal)
Fleming, M.B., Richards, C.M., Walters, C.T. 2017. Decline in RNA integrity of dry-stored soybean seeds correlates with loss of germination potential. Journal of Experimental Botany. 68: 2219-2230. doi: 10.1093/jxb/erx100.

Variation of desiccation tolerance and longevity in fern spores - (Peer Reviewed Journal)
Ballesteros, D., Hill, L.M., Walters, C.T. 2017. Variation of desiccation tolerance and longevity in fern spores. Journal of Plant Physiology. 211:53-62 doi.org/10.1016/j.jplph.2017.01.003.

Changes in barley (Hordeum vulgare L. subsp. vulgare) genetic diversity and structure in Jordan over a period of 31 years Reprint Icon - (Peer Reviewed Journal)
Thormann, I., Reeves, P.A., Thumm, S., Reilley, A.A., Engels, J.M., Biradar, C.M., Lohwasser, U., Börner, A., Pillen, K., Richards, C.M. 2017. Changes in barley (Hordeum vulgare L. subsp. vulgare) genetic diversity and structure in Jordan over a period of 31 years. Plant Genetic Resources. doi:10.1017/S1479262117000028.

Changes in transcript expression patterns as a result of cryoprotectant treatment and liquid nitrogen exposure in Arabidopsis shoot tips - (Peer Reviewed Journal)
Gross, B.L., Henk, A.D., Bonnart, R.M., Volk, G.M. 2016. Changes in transcript expression patterns as a result of cryoprotectant treatment and liquid nitrogen exposure in Arabidopsis shoot tips. Plant Cell Reports. 36:459-470. doi:10.1007/s00299-016-2095-7.

Improved droplet-vitrification and histological studies of cryopreserved shoot tips of cultivated Jerusalem artichoke genotypes - (Peer Reviewed Journal)
Zhang, J., Han, L., Lu, X., Volk, G.M., Xin, X., Yin, G., He, J., Wang, L., Chen, X. 2016. Improved droplet-vitrification and histological studies of cryopreserved shoot tips of cultivated Jerusalem artichoke genotypes. Plant Cell Tissue and Organ Culture. 128:327-334.

Probabilistic viability calculations for cryopreserving vegetatively propagated collections in genebanks - (Peer Reviewed Journal)
Volk, G.M., Henk, A.D., Jenderek, M.M., Richards, C.M. 2016. Probabilistic viability calculations for cryopreserving vegetatively propagated collections in genebanks. Genetic Resources and Crop Evolution. doi:10.1007/S10722-016-0460-6.

Citrus cyopreservation: viability of diverse taxa and histological observations - (Peer Reviewed Journal)
Volk, G.M., Bonnart, R.M., Shepherd, A.N., Yin, Z., Lee, R.F., Polek, M., Krueger, R. 2016. Citrus cyopreservation: viability of diverse taxa and histological observations. Plant Cell Tissue and Organ Culture. doi:10.1007/211240-016-1112-4.

Effect of error and missing data on population structure inference using microsatellite data Reprint Icon - (Peer Reviewed Journal)
Reeves, P.A., Bowker, C.L., Fettig, C.E., Tembrock, L.R., Richards, C.M. 2016. Effect of error and missing data on population structure inference using microsatellite data. bioRxiv. https://doi.org/10.1101/080630.

Seeds capture the diversity of genetic resource collections of Malus sieversii maintained in an orchard - (Peer Reviewed Journal)
Volk, G.M., Henk, A.D., Forsline, P.L., Szewc-McFadden, A.K., Fazio, G., Aldwinckle, H., Richards, C.M. 2016. Seeds capture the diversity of genetic resource collections of Malus sieversii maintained in an orchard. Genetic Resources and Crop Evolution. doi:10.1007/s10722-016-0450-8.

Genotypic and phenotypic changes in wild barley (Hordeum vulgare subsp. spontaneum) during a period of climate change in Jordan - (Peer Reviewed Journal)
Thormann, I., Reeves, P.A., Reilley, A.A., Engels, J.M., Biradar, C.M., Lohwasser, U., Börner, A., Pillen, K., Richards, C.M. 2017. Genotypic and phenotypic changes in wild barley (Hordeum vulgare subsp. spontaneum) during a period of climate change in Jordan. Genetic Resources and Crop Evolution. 64:1295-1312.

Geography of genetic differentiation in the barley wild relative Hordeum vulgare subsp. spontaneum in Jordan - (Peer Reviewed Journal)
Thormann, I., Reeves, P.A., Reilley, A.A., Engels, J.M., Lohwasser, U., Boerner, A., Pillen, K., Richards, C.M. 2016. Geography of genetic differentiation in the barley wild relative Hordeum vulgare subsp. spontaneum in Jordan. PLoS One. DOI:10.1371/journal.pone.0160745.

The value of citrus genebanking - (Trade Journal)
Volk, G.M., Lee, R.F., Krueger, R. 2016. The value of citrus genebanking. Citrograph. 7(4):42-44.

The potential for citrus cryotherapy - (Trade Journal)

Investigating the role of extensin proteins in poplar biomass recalcitrance - (Peer Reviewed Journal)
Fleming, M.B., Decker, S.R., Bedinger, P.A. 2016. Investigating the role of extensin proteins in poplar biomass recalcitrance. BioResources. 11(2):4727-4744.

Historic American apple cultivars: Identification and availability - (Peer Reviewed Journal)
Volk, G.M., Henk, A.D. 2016. Historic American apple cultivars: Identification and availability. Journal of the American Society for Horticultural Science. 141(3):292-301.

Prioritization of Malus accessions for collection cryopreservation at the USDA-ARS National Center for Genetic Resources Preservation Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Jenderek, M.M., Chao, C.T. 2017. Prioritization of Malus accessions for collection cryopreservation at the USDA-ARS National Center for Genetic Resources Preservation. Acta Horticulturae. 1172:267-272 doi: 10.17660/ActaHortic.2017.1172.51.

Preserving oak (Quercus sp.) germplasm to promote ex situ conservation - (Peer Reviewed Journal)
Walters, C.T., Crane, J., Hill, L.M., Michalak, M., Carstens, J.D., Conrad, K.P. 2016. Preserving oak (Quercus sp.) germplasm to promote ex situ conservation. International Oaks. 27:255-266..

Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration - (Peer Reviewed Journal)
Mira, S., Hill, L.M., Gonzalez-Benito, M., Ibanez, M.A., Walters, C.T. 2016. Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration. Journal of Experimental Botany. 67(6):1783-1793.

A strategy to conserve worldwide apple genetic resources: Survey results Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Bramel, P. 2017. A strategy to conserve worldwide apple genetic resources: Survey results. Acta Horticulturae. 1172:99-106 doi: 10.17660/ActaHortic.2017.1172.18.

Recovery patterns, histological observations and genetic integrity in Malus shoot tips cryopreserved using droplet vitrification and encapsulation-dehydration procedures - (Peer Reviewed Journal)
Li, B., Feng, C., Hu, L., Wang, M., Volk, G.M., Wang, Q. 2015. Recovery patterns, histological observations and genetic integrity in Malus shoot tips cryopreserved using droplet vitrification and encapsulation-dehydration procedures. Journal of Biotechnology. 214:182-191.

Genetic diversity of Malus cultivars and wild relatives in the Chinese National Repository of Apple Germplasm Resources Reprint Icon - (Peer Reviewed Journal)
Gao, Y., Liu, F., Wang, K., Wang, D., Gong, X., Liu, L., Richards, C.M., Henk, A.D., Volk, G.M. 2015. Genetic diversity of Malus cultivars and wild relatives in the Chinese National Repository of Apple Germplasm Resources. Tree Genetics and Genomes. 11:106. doi:10.1007/s11295-015-0913-7.

Identification and conservation of apple genetic diversity - (Abstract Only)
Volk, G.M. 2015. Identification and conservation of apple genetic diversity. National Association for Plant Breeders, Pullman, WA. July 28-30, 2015. Pg.21.

Chloroplast heterogeneity and historical admixture within the genus Malus - (Peer Reviewed Journal)
Volk, G.M., Henk, A.D., Baldo, A.M., Fazio, G., Chao, C.T., Richards, C.M. 2015. Chloroplast heterogeneity and historical admixture within the genus Malus. American Journal of Botany. 102(7):1198-1208. DOI: 10.3732/ajb.1500095.

Identification of a highly successful cryopreservation method (droplet-vitrification) for petunia - (Peer Reviewed Journal)
Zhang, J., Huang, B., Zhang, X., Volk, G.M., Xin, X., Yin, G., He, J., Lu, X., Zhou, Y., Chen, X. 2015. Identification of a highly successful cryopreservation method (droplet-vitrification) for petunia. In Vitro Cellular and Developmental Biology - Plants. 51:445-451.

Conservation of the USDA-ARS National Plant Germplasm System Apple Collection using dormant bud cryopreservation - (Abstract Only)
Volk, G.M., Jenderek, M.M., Chao, C.T. 2015. Conservation of the USDA-ARS National Plant Germplasm System Apple Collection using dormant bud cryopreservation [abstract]. XIV Eucarpia Fruit Breeding and Genetics Symposium. Available: http://www.eucarpiafruit2015.org/wp-content/uploads/2014/06/03-poster-list.pdf.

Cryopreservation of in vitro-grown shoot tips of Chinese medicinal plant Atractylodes macrocephala Koidz. using a droplet-vitrification method - (Peer Reviewed Journal)
Zhang, J., Huang, B., Lu, X., Volk, G.M., Xin, X., Yin, G., He, J., Chen, X. 2015. Cryopreservation of in vitro-grown shoot tips of Chinese medicinal plant Atractylodes macrocephala Koidz. using a droplet-vitrification method. CryoLetters. 36(3):195-204.

Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss - (Peer Reviewed Journal)
Walters, C.T. 2015. Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss. Planta. 242(2):397-406.

Three types of rescue can avert extinction in a changing environment - (Peer Reviewed Journal)
Hufbauer, R.A., Szucs, M., Kasyon, E., Youngberg, C., Koontz, M.J., Richards, C.M., Tuff, T., Melbourne, B.A. 2015. Three types of rescue can avert extinction in a changing environment. Proceedings of the National Academy of Sciences. 112(33):10557-10562.

Developing quantitative seed sampling protocols using simulations: A reply to comments from Guja et al. and Guerrant et al. - (Other)
Hoban, S., Strand, A., Fraga, N., Richards, C.M., Schlarbaum, S. 2015. Developing quantitative seed sampling protocols using simulations: A reply to comments from Guja et al. and Guerrant et al.. Biological Conservation. 184 469-470.

Widespread applications of citrus cryopreservation - (Trade Journal)
Volk, G.M. 2015. Widespread applications of citrus cryopreservation. Citrograph. 6(2):42-44.

The vulnerability of US apple (Malus) genetic resources Reprint Icon - (Peer Reviewed Journal)
Volk, G.M., Chao, C.T., Norelli, J.L., Brown, S.K., Fazio, G., Peace, C., McFerson, J., Zhong, G., Bretting, P.K. 2015. The vulnerability of US apple (Malus) genetic resources. Genetic Resources and Crop Evolution. 62(5):765-794. doi:10.1007/s10722-014-0194-2.

Development of a strategy to conserve worldwide apple genetic resources - (Abstract Only)
Volk, G.M., Hummer, K.E., Bramel, P. 2015. Development of a strategy to conserve worldwide apple genetic resources [abstract]. XIV Eucarpia Fruit Breeding and Genetics Symposium. Available: http://www.eucarpiafruit2015.org/wp-content/uploads/2014/07/eucarpia2k15programme.pdf.

Why is intracellular ice lethal? A microscopical study showing evidence of programmed cell death in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum - (Peer Reviewed Journal)
Wesley-Smith, J., Walters, C.T., Pammenter, N., Berjak, P. 2015. Why is intracellular ice lethal? A microscopical study showing evidence of programmed cell death in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum. Annals of Botany. 115(6):991-1000. DOI:10.1093/aob/mcv009.

Genetic diversity in Malus × domestica (Rosaceae) through time in response to domestication - (Peer Reviewed Journal)
Gross, B., Henk, A.D., Richards, C.M., Fazio, G., Volk, G.M. 2014. Genetic diversity in Malus × domestica (Rosaceae) through time in response to domestication. American Journal of Botany. 101(10):1770-1779. DOI: 10.3732/ajb.1400297.

Cryopreservation of Populus trichocarpa and Salix using dormant buds with recovery by grafting or direct rooting - (Peer Reviewed Journal)
Bonnart, R.M., Waddell, J.W., Haiby, K., Widrlechner, M., Volk, G.M. 2014. Cryopreservation of Populus trichocarpa and Salix using dormant buds with recovery by grafting or direct rooting. CryoLetters. 35(6):507-515.

Factors affecting stress tolerance in recalcitrant embryonic axes from four Quercus (Fagaceae) species native to the US or China - (Peer Reviewed Journal)
Xia, K., Hill, L.M., Decorte, A., Li, D., Walters, C.T. 2014. Factors affecting stress tolerance in recalcitrant embryonic axes from four Quercus (Fagaceae) species native to the US or China. Annals Of Botany. 114(8):1747-1759.

Factors affecting stress tolerance in recalcitrant embryonic axes from four Quercus (Fagaceae) species native to the US or China - (Peer Reviewed Journal)
Xia, K., Hill, L.M., Li, D., Walters, C.T. 2014. Factors affecting stress tolerance in recalcitrant embryonic axes from four Quercus (Fagaceae) species native to the US or China. Annals Of Botany. 114(8):1747-1759. DOI: 10.1093/aob/mcu193.

Genetic relationships between wild progenitor pear (Pyrus L.) species and local cultivars native to Georgia, South Caucasus - (Peer Reviewed Journal)
Asanidze, Z., Akhalkatsi, M., Henk, A.D., Richards, C.M., Volk, G.M. 2014. Genetic relationships between wild progenitor pear (Pyrus L.) species and local cultivars native to Georgia, South Caucasus. Flora. 209(9):504-512. DOI: 10.1016/j.flora.2014.06.013.

A global conservation strategy for apple - (Abstract Only)
Volk, G.M., Chao, C.T., Hummer, K.E. 2014. A global conservation strategy for apple [abstract]. 7th International Rosaceae Genomics Conference. p. 28.

Application of genetic and spatial analyses to identify collection priorities for wild Malus species - (Abstract Only)
Volk, G.M., Richards, C.M., Henk, A.D., Baldo, A.M., Fazio, G., Chao, C.T. 2014. Application of genetic and spatial analyses to identify collection priorities for wild Malus species [abstract]. 7th International Rosaceae Genomics Conference. p. 84.

An efficient, widely applicable cryopreservation of Lilium shoot tips by droplet vitrification - (Peer Reviewed Journal)
Yin, Z., Bi, W., Chen, L., Zhao, B., Volk, G.M., Wang, Q. 2014. An efficient, widely applicable cryopreservation of Lilium shoot tips by droplet vitrification. Acta Physiologiae Plantarum. 36(7):1683-1692. DOI: 10.1007/s11738-014-1543-7.

Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication - (Peer Reviewed Journal)
Wang, B., Wang, R., Cui, Z., Li, J., Bi, W., Li, B., Ozudogru, E., Volk, G.M., Wang, Q. 2014. Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication. Biotechnology Advances. 32(3):583-595. DOI: 10.1016/j.biotechadv.2014.03.003.

Genebanking seeds from natural populations - (Peer Reviewed Journal)
Walters, C.T. 2015. Genebanking seeds from natural populations. Natural Areas Journal. 35(1):98-105. DOI: 10.3375/043.035.0114.

Strategies for improved efficiency when implementing plant vitrification techniques - (Peer Reviewed Journal)
Volk, G.M., Shepherd, A., Bonnart, R.M. 2014. Strategies for improved efficiency when implementing plant vitrification techniques. Acta Horticulturae. 1039:85-89.

Plant shoot tip response to treatment with plant vitrification solution 2 - (Peer Reviewed Journal)
Volk, G.M., Henk, A.D., Bonnart, R.M., Shepherd, A., Gross, B.L. 2014. Plant shoot tip response to treatment with plant vitrification solution 2. Acta Horticulturae. 1039:81-84.

Extreme biology: probing life at low water contents and temperatures - (Peer Reviewed Journal)
Walters, C.T. 2014. Extreme biology: probing life at low water contents and temperatures. Acta Horticulturae. 1039:49-56.

Somatic embryogenesis and organogenesis from cryopreserved shoot tips of Lilium Oriental hybrid ‘Siberia’ - (Peer Reviewed Journal)
Yin, Z., Chen, L., Bi, W., Volk, G.M., Wang, Q. 2014. Somatic embryogenesis and organogenesis from cryopreserved shoot tips of Lilium Oriental hybrid ‘Siberia’. Acta Horticulturae. 1039:193-200.

Seed biology of tree species and strategies for ex situ conservation - (Proceedings)
Walters, C.T. 2013. Seed biology of tree species and strategies for ex situ conservation. In: Proceedings of the International Symposium on Breeding and Utilization of Forest Special Purpose Trees, September 9-12, 2013, Suwon, Korea. p.23-35.

Extreme biology: probing life at low water contents and temperatures - (Abstract Only)
Walters, C.T. 2013. Extreme biology: probing life at low water contents and temperatures. International Society for Horticultural Science Meeting. p.19.

Cryopreservation of pecan pollen for long-term storage - (Abstract Only)
Volk, G.M., Walters, C.T., Waddell, J., Grauke, L.J. 2013. Cryopreservation of pecan pollen for long-term storage. Meeting Abstract. Second International Symposium on Plant Cryopreservation, p.106.

Cryopreservation of citrus in the USDA-ARS national plant germplasm system collection - (Abstract Only)
Bonnart, R.M., Shepherd, A., Lee, R.F., Krueger, R., Volk, G.M. 2013. Cryopreservation of citrus in the USDA-ARS national plant germplasm system collection. Meeting Abstract. 2nd International Symposium on Plant Cryopreservation. p.62.

Cryopreservation of vitis shoot tips from diverse species - (Abstract Only)
Shepherd, A., Bonnart, R.M., Volk, G.M. 2013. Cryopreservation of vitis shoot tips from diverse species. Meeting Abstract. Second International Symposium on Plant Cryopreservation, p.103.