Location: National Germplasm Resources Laboratory2015 Annual Report
1a. Objectives (from AD-416):
1) Apply the expert knowledge of project staff, Crop Germplasm Committees (CGCs), National Plant Germplasm System (NPGS) curators, scientific literature, and GIS and other analyses of national and international databases to identify and prioritize strategic gaps in NPGS crop collections, and facilitate the acquisition of the genetic resources needed to fill those gaps. 1a) Conduct systematic and ecogeographical analyses to identify key gaps in NPGS collections, prioritize acquisitions needed to fill those gaps, and identify opportunities for acquisition. 1b) Develop linkages between NPGS genebanks and federal and state landholding agencies to preserve in in situ reserves native taxa related to the crop species. 2) Maintain and augment GRIN-Taxonomy for Plants, an online database for validated, standard botanical and cultivated scientific plant names, taxonomic classifications, and associated data. Expand the nomenclatural, classificatory, and ecogeographical information available for crop wild relatives. 2a) Provide accurate scientific plant names in GRIN/GRIN-Global, incorporating new data on classification, synonymy, geographical distributions, economic impacts, and common names that reflect current literature. 2b) Promote global usage of GRIN taxonomic data among genetic resource managers and other agricultural workers. 2c) Expand GRIN taxonomic data on wild relatives of crops. 3) Foster plant genetic resource conservation and sustainable use by facilitating the international export of NPGS germplasm, and by establishing innovative international partnerships to enhance plant genetic resource conservation capacities. Establish and maintain partnerships in developing countries that provide access to their plant genetic resources, both ex situ and in situ, by NPGS-funded plant explorers. These partnerships are to enhance donor nations’ capacity to conserve, document, and use genetic resources. 3a) Establish and maintain partnerships in developing countries which provide access to their plant genetic resources, both ex situ and in situ, by NPGS-funded plant explorers. These partnerships are to enhance donor nations' capacity to conserve, document, and use genetic resources. 3b) In collaboration with the USDA-APHIS and DHS/Customers and Border Patrol/Airport Inspection Service, facilitate the import of plant genetic resources and accompanying documentation from donor countries, and the export of NPGS germplasm to other countries, as part of international plant exhanges. 4) Serve as coordinator and secretariat for the 42 Crop Germplasm Committees who collectively provide technical input for NPGS plant genetic resource management plans and priorities, and catalyze a greater awareness of current genetic resource management efforts for these crops.
1b. Approach (from AD-416):
Crops will be reviewed for germplasm gaps in the general order of economic value. On a crop-by-crop basis all taxa related to the crop under study and their relative importance as potential donors of genes for crop improvement will be identified. Gaps will then be identified and prioritized. This list will then be reviewed to determine if some or all of them can be acquired through exchange or if collection from in situ populations is needed. Once geographic areas are identified from which collections are needed, countries in which access to genetic resources is likely will be determined. The assessment will be sent to the NPGS crop curators and the appropriate CGCs for validation and modification. To maintain GRIN-Taxonomy for Plants the latest taxonomic literature will be monitored through routine searches to detect any changes to existing taxonomic classification or nomenclature for possible adoption in GRIN/GRIN-Global. All data will be documented by references cited in GRIN. Employing the definitions of genetic-relative classes, CWR classification will be developed for each crop based on thorough review of all pertinent taxonomic and phylogenetic literature, as well as similar review of the plant-genetic, plant-breeding, or other crop-science literature. Working directly with representatives of the federal and state land-managing agencies, this project will develop formal non-funded, interagency agreements to establish in situ reserves for target populations of crop wild relatives on land protected by the agencies. Non-monetary benefits will be negotiated and provided to countries that give prior informed consent for access to their plant genetic resources. The nature of such small-scale projects will be highly variable, but possibilities include funding of an evaluation and regeneration project in the host country for the collected germplasm, local or international training in genebank related activities, and the purchase of laboratory, field, or data processing equipment for the genebank or host institution. Animal and Plant Health Inspection Service (APHIS) issued permits for non-regulated nursery stock, small seed lots without a phytosanitary certificate, and herbarium specimens will be maintained to facilitate the import of germplasm for the NPGS. Instruction sheets on Import/Export procedures and proper shipping of germplasm to the NPGS will also be regularly updated and made available to NPGS personnel. Regular communications with Department of Homeland Security Customs and Border Protection and APHIS on importing plants will also be maintained. Export of NPGS germplasm to researchers and educators worldwide will be supported by facilitating the agriculture inspections and issuance of phytosanitary certificates by APHIS, and shipping the germplasm to the final destination. This project will also coordinate and participate in the annual meetings of the CGC Chairs, maintain documentation of the activities of CGCs (minutes, membership rosters, crop vulnerability reports, etc.), and serve as a liaison between CGCs and ARS leadership to highlight issues and concerns related to plant genetic resources.
3. Progress Report:
Twelve plant explorations were coordinated by NGRL in FY 15. In cooperation with host country institutions, explorations took place in Armenia and Georgia. Ten explorations also took place in the United States. Germplasm samples collected included genetic resources of apple, ash, beet, potato, sunflower, switchgrass, and herbaceous and woody ornamentals. These accessions are new sources of genetic diversity that will soon be available in the National Plant Germplasm to scientists worldwide for use in crop breeding and other research. The existing collaborative projects in Albania, Georgia, and Azerbaijan were maintained and supported. Numerous adjustments were made to GRIN taxonomy data, including the addition of more than 10,400 distribution, 10,400 literature, 130 common name, and 440 economic use records. Modifications to more than 9,300 existing records have also been made in preparation for the migration of these data to the GRIN-Global platform. Editing work was completed on over 50 nomenclature proposals for the journal Taxon. The hardcopy edition of the Appendices for the 2012 International Code of Nomenclature for algae, fungi and plants was published from a database created for this purpose. Over 145 major and minor crops in 77 genera now have a classification of their crop wild relatives compiled and publicly available on GRIN. Between October 1, 2014 and June 23, 2015 PEO assisted in the distribution of 29,368 NPGS accessions to researchers in 59 different countries throughout the world, and in the importation of shipments of germplasm from different foreign countries for U.S. researchers and the National Plant Germplasm System. A joint strategic framework for collaboration between ARS and the U.S. Forest Service on complementary in situ and ex situ conservation of crop wild relatives was approved and released. A project to select and establish in situ reserves for cranberry (Vaccinium macrocarpon) on National Forests proceeded with tissue and germplasm sampling of 27 populations on seven National Forests. Tissue samples are currently undergoing analysis for identification of genetically unique and\or diverse populations. Germplasm has been sent to the USDA National Clonal Repository in Corvallis, Oregon.
1. Plant explorations were conducted to obtain documented genetic resources for the U.S. National Plant Germplasm System. Twelve plant explorations were coordinated by ARS researchers in Beltsville, Maryland, in FY 15. In cooperation with host country institutions, explorations took place in Armenia and Georgia. Ten explorations also took place in the United States. Germplasm samples collected included genetic resources of apple, ash, beet, potato, sunflower, switchgrass, and herbaceous and woody ornamentals. These accessions are new sources of genetic diversity that will soon be available in the National Plant Germplasm System to scientists worldwide for use in crop breeding and other research.
2. Internationally recognized resource for plant taxonomy of agricultural plants. ARS scientists in Beltsville, Maryland, updated approximately 4,000 taxon records were updated in GRIN Taxonomy during FY 15. Nearly 3,000 of these were new additions to GRIN. Some of these included accommodating additional accessions from the Seeds of Success project of the Bureau of Land Management and the ARS Western Regional Plant Introduction Station. Correct classification and naming of germplasm accessions is critical for optimally curating and utilizing them. Many genebanks, research organizations and individuals worldwide now routinely rely on the information in GRIN Taxonomy, and records have been added to GRIN Taxonomy during FY 15 to accommodate the needs of the National Agricultural Library, the International Union for the Protection of New Varieties of Plants (UPOV), the International Maize and Wheat Improvement Center (CIMMYT), and the Czech National Genebank. Additionally, data on wild relatives for 145 major and minor crops is now publicly available in GRIN Taxonomy. These data identify and classify over 3,600 primary, secondary, tertiary, and graft-stock genetic relatives for these crops, and are searchable by various criteria.