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United States Department of Agriculture

Agricultural Research Service

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Research Project: Development of a TaqMan Array Card for Multiplex Detection of Citrus Pathogens by Real-Time PCR

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-011-06-T
Project Type: Trust

Start Date: Jan 1, 2013
End Date: Sep 30, 2016

Develop a custom multiplex TaqMan® Array card as a standardized real-time polymerase chain reaction assay for simultaneous detection of RNA and DNA citrus pathogens of regulatory importance to the California citrus nurseries.

Use and modify commercial kits which capture nucleic acid on magnetic beads during robotic extraction to yield high quality total nucleic acid; utilize existing literature and validate or improve real-time PCR detection of specific RNA and DNA targets; develop a custom micro-fluidic card with multiple pathogen target signatures (primer sets) for amplicon reaction with TaqMan® probes; use a block specifically designed to conduct qPCR with 384-well microfluidic cards.

Last Modified: 2/23/2016
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