Location: Plant Physiology and Genetics Research
Project Number: 2020-21410-006-05-A
Project Type: General Cooperative Agreement
Start Date: May 1, 2013
End Date: Apr 30, 2017
To 1) identify, genotype, and impute genetic markers in diverse Brassica napus accessions with non-reference and reference genome approaches; 2) construct a sequence variant map for diverse Brassica napus; 3) genotype and construct genetic linkage maps for recombinant inbred line populations; and 4) conduct genome-wide association studies and linkage analysis of agronomic and biofuel traits in Brassica napus.
Genotyping-by-sequencing data collected on more than 1800 Brassica napus accessions and recombinant inbred lines from the USDA-ARS GRIN collection and ongoing ARS and academic breeding programs will be analyzed with computational biology approaches to simultaneously discover and genotype highly-dense genetic markers at genome-wide coverage, followed by the construction of sequence variant and genetic linkage maps. Statistical genetics approaches will be used to identify marker-trait associations for direct application in the development of breeding lines with improved oil production and quality for the hydrotreated renewable jet (HRJ) fuel industry.