Location: Foreign Animal Disease Research
Project Number: 8064-32000-057-49-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Feb 1, 2013
End Date: Jul 31, 2014
This collaborative research project seeks to determine whether different routes of application of emergency oil emulsion Foot-and-Mouth Disease (FMD) vaccines lead to varying efficacy in Merino sheep and prevent the establishment of the carrier state. Specific objectives include: 1. Determine the optimal route of inoculation for sheep to induce reproducible clinical disease. 2. Determine the optimal route of vaccine application in sheep using emergency, high potency double oil emulsion serotype O FMD vaccine. 3. Determine the protection afforded by the best route as determined above with heterologous live virus challenge. 4. Determine if vaccination prevents the establishment of a carrier state. 5. Determine what dose of vaccine best prevents the establishment of a carrier state.
1. A comparison study of intranasal instillation and aerosol infection using a commercially available aerosol delivery system to determine the most reproducible way of infecting Merino sheep will be conducted. 2. A delivery study utilizing decreasing doses of emergency serotype O vaccine via the intra-muscular and intra-dermal routes will be conducted. The immune responses of each will be studied using in vitro assays. 3. In vivo vaccination studies will be conducted using the best methods determined above and challenge with a heterlogous virus to determine vaccine efficacy. 4. To determine carrier state activity, vaccinated and infected sheep will be studied for at least 35 days post infection. Studies will also include minimal protective dose.