Location: Crop Genetics and Breeding Research
Project Number: 6048-21000-026-000-D
Project Type: In-House Appropriated
Start Date: May 27, 2013
End Date: Mar 5, 2018
1. Develop peanut germplasm with improved drought tolerance and reduced preharvest aflatoxin contamination (PAC). 2. Develop high oleic acid peanut germplasm with improved resistance to diseases and nematodes, such as the peanut root-knot nematode. 3. Develop and conduct phenotypic evaluations of recombinant inbred line (RIL) populations to aid in the development of molecular markers, and use these to develop effective marker assisted selection techniques in peanut.
1. Breeding populations will be developed by hybridizing high yielding cultivars with sources of resistance to preharvest aflatoxin contamination and sources of resistance to drought. These populations will be evaluated under field conditions with drought and heat stress imposed by covering the entire test area with a mobile greenhouse. Aflatoxin contamination of the subsequent yield will be determined using the immunoaffinity column fluorometer method. Progeny will be selected based on relatively low aflatoxin contamination and/or relatively high pod yields. 2. Breeding populations will be developed by hybridizing cultivars with high oleic acid with high yielding breeding lines with resistance to the peanut root-knot nematode and tomato spotted wilt virus. Marker assisted selection will be utilized to select early generation progeny that are homozygous for nematode resistance and the high oleic characteristic. Selection in later generations will focus on field resistance to tomato spotted wilt virus, high yield, and other agronomic characteristics. Sixteen structured recombinant inbred line IRIL) populations will be developed using parents that were selected to maximize genetic diversity while meeting practical breeding objectives. In-depth phenotyping and genotyping of these populations will be conducted to identify genetic markers that can be used in peanut cultivar development.