1a. Objectives (from AD-416):
Generate a whole cotton transcriptome profile of water deficit stress responsive genes.
1b. Approach (from AD-416):
We will generate a comprehensive whole transcriptome profile of genes differentially expressed in response to field water deficit stress. Well-watered and water deficit stress conditions will be maintained using above ground drip irrigation under naturally occurring field conditions. In the first field experiment, we will focus on identifying genes differentially expressed in response to water deficit stress occurring during fiber development. In the second field experiment, we will focus on identifying genes differentially expressed in response to water deficit stress across a gradient of plant stress levels.
3. Progress Report:
This project is related to objective 1 of the in-house project: to develop genetic resources and cropping practices that increase cotton water-use efficiency. Water deficit stress is known to be a major inhibitor of environmentally and economically sustainable cotton production systems. The primary objective of this cooperative research project is to identify genes with expression sensitivity to water deficit stress occurring during fiber elongation. In 2013, a single cultivar is being grown in the field. At the onset of flowering, well-watered and water deficit stress treatments will be imposed. A time-gradient of developing boll samples will be collected for monitoring gene expression during fiber elongation. Following downstream gene expression analyses, the study will provide a catalog of candidate genes differentially expressed in response to water deficit stress occurring during fiber elongation. These candidate genes will serve cotton breeding programs as genetic targets for improving cotton’s productivity under water deficit stress.