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United States Department of Agriculture

Agricultural Research Service

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Research Project: Determination of post fumigation recovery of soil microbial communities over time

Location: Water Management Research

Project Number: 2034-13000-011-18-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2012
End Date: Jun 30, 2015

1) Determine the recovery of microbial community structure after fumigation with time. 2) Examine the effect of fumigants on both target and non-target organisms in soil. 3) Compare these non-target organisms in fumigated vs. non-fumigated control sites.

Soil samples will be collected from different aged fumigated sites from calla lily field in Moss Landing, CA. Fumigated sites ranging in age approximately from few months to 30 years, will be selected. Non-fumigated fields will be used as a control. From each site, four transects will be identified and from each transect, soil samples will be collected from 4 different locations and 2 depths (0-5 cm and 5-15 cm). Phospholipid fatty acid (PLFA) analysis will be used to study the microbial community structure recovery for non-target organisms. Determination of Fusarium oxysporum and Pythium spp. populations will be done using dilution plating on Komada’s and P5ARP medium respectively.

Last Modified: 10/18/2017
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