Project Number: 6034-21000-016-02-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Oct 1, 2012
End Date: Sep 30, 2014
To distinguish live from dead cells of Candidatus Liberibacter asiaticus, presumed causal agent of citrus huanglongbing disease, in citrus tissue.
The use of two DNA intercalating dyes (Ethidium Monoazide and Prodidium Monoazide) will be used to degrade DNA from dead cells while not affecting DNA contained in live cells, following treatment, DNA will be extracted and used in qPCR analysis; only DNA from live cells should amplify. Results with and without dye will be compared, parameters optimized and tested in citrus samples expressing various levels of HLB symptoms.