Location: Crop Bioprotection Research
Project Number: 5010-22410-015-01-N
Project Type: Nonfunded Cooperative Agreement
Start Date: Apr 1, 2013
End Date: Dec 31, 2014
1) Evaluate the efficacy of new formulations of Fusarium head blight antagonist Cryptococcus flavescens OH 182.9 3C against FHB and DON under field conditions, 2) characterize the biogeography and determine the diversity of C. flavescens isolated from non-inoculated wheat fields, and 3) determine the survival and dissemination of 3C inoculant through the food system.
Commercially suitable formulations of Cryptococcus flavescens OH 182.9 3C will be produced by Verdesian Life Sciences from biomass of the strain supplied by the ARS collaborator. The best 4 products produced will be field tested, in the first year of studies, on a FHB susceptible and a moderately resistant cultivar by Ohio State collaborators in Wooster, OH and by the ARS collaborator in Peoria, IL. Wheat plants will be scored for FHB disease parameters and the deoxynivalenol content of grain. In a second year of field studies, results will be confirmed for superior formulations and inferior formulations substituted into a wheat field trial in each location. For objective 2, Ohio State collaborators will use a qPCR assay they developed previously to quantify the incidence and population size of C. flavescens in wheat and barley fields at multiple levels of scale. Primers derived from the sequence of the 3C genome will be used to evaluate the diversity of natural populations of C. flavescens to determine their relatedness to 3C. For objective 3, similar primers and techniques will be used to quantify natural populations of C. flavescens and strain 3C in wheat grain harvested from 3C-inoculated and non-inoculated fields.