Project Number: 6612-32000-005-07-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Sep 1, 2012
End Date: Aug 30, 2013
1. Determine the incidence of Salmonella, E. coli and enterococci in swine fecal samples in the United States. 2. Participate in the characterization of isolates at the phenotypic and genotypic level.
Fecal samples will be sampled using microbiological culture techniques to determine bacterial presence. Polymerase chain reaction (PCR) and Sensititre broth microdilution plates will be used to determine select antimicrobial attributes; pulsed field gel electrophoresis (PFGE) will be used to determine relatedness.