Location: Food and Feed Safety Research
Project Number: 3091-32000-033-01-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 17, 2012
End Date: Sep 16, 2017
1) Establish the duration of infection of Salmonella within the peripheral lymph nodes of cattle as influenced by serotype; 2) Develop an understanding of cellular tropism across various serotypes with and without interventions; and 3) Conduct rapid and well-controlled evaluation of candidate interventions designed to reduce the duration of infection within lymph nodes of cattle.
Studies will be conducted on dairy calves, utilizing a parent project-developed transdermal model for peripheral lymph node uptake of Salmonella. The first study will examine the duration of infection of Salmonella in several peripheral lymph nodes. Using the transdermal model, different Salmonella serotypes will be administered to individual animals to determine if duration of infection is influenced by serotype. Utilizing information regarding the molecular characterization of Salmonella sub-populations that invade lymph nodes, a second study will compare the response of Salmonella following intradermal administration of vaccine interventions. Finally, results from the first two sets of experiments will be utilized to design and test interventions for their ability to prevent and eliminate lymph node colonization by Salmonella following intradermal administration.