Location: Plant Germplasm Preservation Research
Project Number: 3012-21000-014-05-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Oct 1, 2011
End Date: Sep 30, 2016
The primary goal of this research is to provide a security back-up of critical California Citrus genetic resources. Objectives 1) Improve upon the existing cryopreservation procedure (developed in the Volk Laboratory) for clones representing diverse Citrus species that use budwood directly from the screenhouse as source materials. 2) Perform long-term cryopreservation exposure experiments to confirm viability. 3) Evaluate a subset of recovered shoot tips to ensure materials are true-to-type.
This research project will develop and implement technologies to cryopreserve critical clones included in the Citrus Clonal Protection Program in Riverside, California. Preliminary data suggests that diverse Citrus clones can be placed into long-term liquid nitrogen storage and then recovered on-demand using modified shoot tip micrografting techniques. Cryopreserved shoot tips can remain viable for dozens of years. This research project will establish a long-term cryoexposure experiment to confirm viability after extended lengths of LN exposure. The project will also identify optimal cryoprotectant exposure times and source plant materials for improved cryopreservation success. These experiments are critical for the implementation of Citrus cryopreservation methods as a collection conservation strategy. It is critical that materials be backed-up for security purposes before California Citrus genetic resources are threatened by Huanglongbing and other diseases or natural threats.