1a. Objectives (from AD-416):
Develop diagnostic resources to support identification and eradication of pest fruit flies.
1b. Approach (from AD-416):
Generate fruit fly specimen inventory, characterize the inventory using molecular and morphological techniques, and develop diagnostic tools based on these characters.
3. Progress Report:
Goals for this Agreement include developing tools to identify the source of intercepted medfly and oriental fruit fly in mainland United States, develop tools for distinguishing Vienna mass reared line medfly from wild using tsl/wp based markers, and define populations of oriental fruit fly from high density single-nucleotide polymorphism (SNP) datasets which contributes to objective 4 of the in-house project, "Assess the efficacy and quality of laboratory-reared insects used in sterile insect techniques (SIT) and natural enemies for control of fruit flies and other tropical plant pests of quarantine significance, and determine factors limiting their effectiveness". This new Specific Cooperative Agreement (SCA) with University of Hawaii, Manoa, is focused on developing new resources to help in fruit fly exclusion and eradication. Specifically, tools to identify the source of intercepted medfly or oriental fruit fly are being developed as well as tools for distinguishing Vienna lines of medfly from wild sources (Vienna specific markers). In addition, the invasion history of prior interceptions of medfly and oriental fruit fly is being analyzed. To date, a protocol for multigene sequencing using a combination of dynamic arrays and high throughput sequencing has been developed for both medfly and Oriental fruit fly. This data is being used to evaluate the invasion history of these flies. In addition, in order to identify marker in Vienna lab lines, a crossing experiment has been performed between Vienna 7 lab line flies from California Department of Food and Agriculture (CDFA) rearing facility and an inbred lab line of medfly maintained at USDA Pacific Basin Agricultural Research Center (PBARC), Hilo, Hawaii. In this F1 backcross design, we have generated an F4 population that exhibits the white pupae and temperature sensitive lethal traits present in female Vienna 7 flies, despite being backcrossed to the inbred lab line. This line is being used now for construction of a linkage map utilizing reduced representation sequencing methods.