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United States Department of Agriculture

Agricultural Research Service

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Research Project: Production of Antibodies to Detect for Viruses from the Grapevine Leafroll Complex by Elisa

Location: Horticultural Crops Research

Project Number: 2072-21220-002-18-G
Project Type: Grant

Start Date: Sep 11, 2012
End Date: Sep 10, 2017

Objective:
To produce antibodies against bacterially expressed capsid proteins of five viruses from the leafroll complex to be able to develop triple-antibody sandwich (TAS) ELISA assays.

Approach:
Polyclonal antibodies produced will be tested using protocols established at University of Idaho, Moscow, and USDA-ARS, Corvallis, against the reference isolates of GLRaV-1, -2, -4, and -5 maintained in grapevine hosts at Corvallis, OR. Investigate broad spectrum antibodies against different strains of GLRV-3. TAS-ELISA test protocols developed will be validated on field samples collected in Nez Perce and Latah counties. Amendment 4 adds funds to continue work on antibodies specific to GLRV-2 and a broad spectrum antibodies against different strains of GLRV-3.

Last Modified: 2/23/2016
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