Location: Horticultural Crops Research
Project Number: 2072-21220-002-18-G
Project Type: Grant
Start Date: Sep 11, 2012
End Date: Sep 10, 2017
To produce antibodies against bacterially expressed capsid proteins of five viruses from the leafroll complex to be able to develop triple-antibody sandwich (TAS) ELISA assays.
Polyclonal antibodies produced will be tested using protocols established at University of Idaho, Moscow, and USDA-ARS, Corvallis, against the reference isolates of GLRaV-1, -2, -4, and -5 maintained in grapevine hosts at Corvallis, OR. Investigate broad spectrum antibodies against different strains of GLRV-3. TAS-ELISA test protocols developed will be validated on field samples collected in Nez Perce and Latah counties. Amendment 4 adds funds to continue work on antibodies specific to GLRV-2 and a broad spectrum antibodies against different strains of GLRV-3.