1a. Objectives (from AD-416):
1. Cloning and functional determination of virulent genes of Candidatus Liberibacter solanacearum. 2. Construct and evaluate transgenic potato to suppress putative virulent genes of Candidatus Liberibacter solanacearum.
1b. Approach (from AD-416):
1. Establish ZC-infected plants through graft inoculation (or by psyllid transmission) and conduct the pathogenicity evaluation ZC-infected potato plants under greenhouse and field growing conditions (by collaborator). 2. Perform genome wide sequence analysis, identify and clone virulent genes of Candidatus Liberibacter solanacearum (by ARS Scientist). 3. Conduct molecular characterization and functional analyses of target genes using a heterologous gene expression system (by ARS Scientist). 4. Develop and evaluate zebra chip resistant potato through antisense and/or RNAi techniques (by collaborator and ARS scientist).
3. Progress Report:
Results from this study are in support of objective 2 of the parent project. The goal of the project is to identify potential virulence-associated genes in potato zebra chip bacterium, ‘Candidatus Liberibacter’ solanacearum and develop a target specific gene silencing technique to control zebra chip disease. This specific cooperative agreement was established on July 24, 2012. ARS scientists at Parlier, California and cooperating researchers have initiated research.