Location: Foreign Animal Disease Research
Project Number: 8064-32000-061-10-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Apr 1, 2012
End Date: Apr 18, 2015
The goal of this project is to conduct phylogenetic, temporal and spatial analysis and modeling of viral strains circulating in Central Asia, Southeast Asia and Africa. Specific objectives include: 1. Gain a better understanding of the epidemiology of Foot-and-Mouth Disease Virus (FMDV) strains circulating in selected endemic regions of Asia and Africa. 2. Determine the phylogenetic relationships of FMDV strains in endemic settings and the nature and extent to which such relationships are associated with epidemiological factors or the settings. 3. Determine the antigenic relationship of FMDV strains circulating in select regions with currently available vaccine strains.
1. Data from FMD outbreaks and active (non-outbreak) surveillance of infected populations will be collected through ongoing field activities in Vietnam, Pakistan and Cameroon. The samples will be tested by rRT-PCR and virus isolation. Sequencing will be conducted and the data generated will be incorporated with known epidemiological data to determine differences between spatial and temporal variables in an effort to determine virus distribution and gain an understanding of viral – host genomic factors. A longitudinal field study will be conducted to determine the viral and host genomic factors influencing viral persistence in local livestock to gain a better understanding of the mechanisms of FMDV persistence. 2. Genetic characterization of viral strains obtained over a 2 -3 year period in three endemic regions of Africa, Central and Southeast Asia will be conducted. The strain information will be coupled with associated geographic and temporal data, case history and animal demographics to determine associations between the pair-wise genetic distances of FMDV isolates and epidemiological factors. 3. Vaccine-strain matching studies will be conducted, which may lead to the recommendation of adding new vaccine antigens to the existing vaccine bank. Determination of the ratio of heterologous vs. homologous titers will be conducted by neutralization with reference sera to relevant predicted vaccine strains and by ELISA. Antigenic cartography will be conducted using serological data to visualize and quantify antigenic relationships among viruses and sera.