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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Research Project #423116


Location: Cool and Cold Water Aquaculture Research

2013 Annual Report

1a. Objectives (from AD-416):
The objectives of this project are to characterize a recently emerging Weissela sp. bacterium by whole genome sequencing. Use sequences to identify potential virulence factors as well as diagnostic and vaccine targets.

1b. Approach (from AD-416):
Sequencing will be accomplished using an Ion Torrent instrument to rapidly sequence and produce a high-quality draft assembly at approximately 30x coverage of the novel Weissela sp. bacterium and thus rapidly determine potential virulence factor genes of the bacterium.

3. Progress Report:
Novel Weissella sp. bacteria have recently been reported to be associated with disease outbreaks in cultured rainbow trout at commercial farms in China, Brazil and most recently in the United States. Comparison of the genome sequences of the US, Brazilian and Chinese isolates is necessary to understand the evolutionary relationship between the isolates and may additionally provide insight into the recent emergence of this pathogen. As a basis for these comparisons, and to identify putative virulence genes, we sequenced the genome of Weissella sp. NC36, a representative isolate from the United States outbreak. The finished genome is 1,358,343 base pairs. Comparative analysis highlighted several putative virulence factors, which were not observed to be present in any of the other sequenced Weissella genomes.

4. Accomplishments