Location: Plant Physiology and Genetics Research
Project Number: 2020-21410-006-01-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Oct 1, 2012
End Date: Aug 31, 2016
To 1) construct a high-density marker map for 800 diverse Brassica napus accessions from a world collection being grown by the University of Idaho and the USDA-ARS; 2) deliver processed genomic sequence to the USDA-ARS Soybase for further processing and public release; and 3) provide additional genomic sequencing as needed to validate marker/gene assignments.
Leaf materials for DNA sequencing will be provided to Keygene company for DNA extraction, followed by genotyping-by-sequencing on the 800 accessions. Web based tools will be used to process sequence information, and deliver via internet to USDA-ARS Soybase for development of a public Brassica napus genomic sequence database. To assist genome wide association mapping strategies, Keygene will provide additional sequencing services as needed.