Project Number: 0210-22310-004-51-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jun 15, 2012
End Date: Jun 15, 2014
1. Expand area of AI/NDV surveillance from Novosibirsk region to the Eastern Siberia. Collect and screen up to 600 samples monthly from poultry and wild birds. 2. Obtain valid data on dynamics of influenza and Newcastle Disease virus distribution in various avian groups. 3. Obtain valid data on infectivity and pathogenic properties of the different avian.influenza viral strains isolated by introducing them into wild bird models and poultry. 4. Establish sample exchange between NSU and collaborative foreign institutions in accordance with the laws of both countries. 5. Share obtained data with international community via workshops, conference presentations and publications.
Under the proposed project, the team of trained virologists and ornithologists will be collecting cloacal and oropharyngeal swabs and serum samples from wild birds and poultry in Eastern Siberia. The samples will include wild migratory aquatic birds, nestling on the lakes and rivers as well as poultry samples (chicken, geese, and ducklings) taken from the commercial poultry farms and back small village holdings. The obtained samples will be screened for Influenza viruses as well as Newcastle Disease viruses (NDV) and if influenza is found, they will be further subtyped as H5, H7, H9 and other subtypes. Screening of the samples will be performed at the Division of Emerging Zoonotic Diseases at Vector and the Department of Natural Science at Novosibirsk State University. Samples, identified as positive, will be investigated further with US collaborators and the obtained results shared with the international community. Another important aspect of this project will involve the investigation of the molecular markers of pathogenicity as well as pathogenicity mechanisms for recently isolated HPAIV, using wild water birds and poultry as models. This will help to understand the behavior of the virus in its natural reservoirs, and it will highlight the role of migrating wild water birds and their role as long-distance vectors for virus transmission around the world.