Location: Cool and Cold Water Aquaculture Research
Project Number: 8082-32000-005-06-A
Project Type: Cooperative Agreement
Start Date: Jan 15, 2012
End Date: Mar 31, 2014
1) Determine if differences in the phagocytic response to F. psychrophilum exists in macrophages and neutrophils isolated from ARS-Fp-R, -C, and –S lines. 2) Determine if antibody-producing (B) lymphocytes from the major tissues of these trout respond differentially to F. psychrophilum vs. control E.coli LPS. 3) Determine if differential cellular and cytokine mechanisms are involved in the observed differences in the antibody responses to T-independent and T-dependent antigens in ARS-Fp-R, -C, and –S lines.
Generally, via in vitro or in vivo analyses of ARS-Fp-R, -C, and-S lines: 1) The ability of phagocytic cells to uptake and destroy live F. psychrophilum will be quantitatively determined using fluorescence microscopy and luminescence assays. 2) In vitro cultures of leukocytes from various immune tissues will be stimulated with LPS from F. psychrophilum and their relative abilities to develop antibody-producing B cells will be assessed. These studies will be coordinated with scientist who will be conducting cytometric characterization of these cells. 3) Trout of all three strains will be immunized with T-dependent and T-independent antigens and ex vivo and in vivo analyses of antibody production and antibody-producing cell development ascertained the effects of splenectomy on the relative ability to generate a productive antibody response will be compared between strains to determine the role splenomegaly may play in resistance.