1a. Objectives (from AD-416):
1. Determine if scald risk assessment tools indicate when delayed CA imposition leads to high scald risk and high scald incidence. 2. Indicate if and when scald risk is high during CA storage based on risk assessment tools and determine if storage conditions can be changed to alter biomarker levels and scald incidence. 3. Assess effectiveness of scald-risk assessment tools in pilot scale and commercial CA storages
1b. Approach (from AD-416):
Our previous work screening hundreds of natural chemicals in apple peel during scald development has revealed those with potential for use as biomarker-based scald risk assessment tools. Further validation is needed to reveal whether tools will aid in commercial storage and supply chain management decisions by monitoring whether CA storage conditions or crop protectant usage is sufficient to prevent superficial scald. We initially developed and validated this technology by measuring peel chemistry changes related to storage stress during the scald development period of fruit stored in both air and CA. We propose continuing this work by testing additional CA conditions in a laboratory setting as well as in pilot or commercial settings. Proposed objectives complement or extend objectives outlined in our existing WTFRC project as well as our recently awarded federal Specialty Crops Research Initiative (SCRI) project to continue to develop biomarker-based storage management tools for superficial scald and other key postharvest disorders
3. Progress Report:
This project relates to objective 2 of the associated in-house project which seeks to identify factors that influence postharvest fruit quality and development of market limiting physiological disorders. Biomarker evaluation experiments for all disorders, were completed. Transcriptomic and metabolomics evaluation of all of the disorders was completed. This provides both a global snapshot of metabolism during the developmental phase of disorder development that will be used to identify key biochemicalpathways and genes associated with disorder development. Validation of biomarker based tools for superficial scal risk assessment in commercial settings indicated similar results to previous lab scale experiments. The results may provide commercially usable risk assessment tools to assist industry in making marketing and storage decisions.