1a. Objectives (from AD-416):
Determine the effect of supplementation with sericea lespedeza pellets to poultry on gastrointestinal parasites and pathogens.
1b. Approach (from AD-416):
This experiment will be conducted at the USDA, ARS (PPPSRU) in Fayetteville, Arkansas. At 14 days of age, broilers will remain uninfected, or be artificially infected with two challenge levels of Ascaridia galli larvae (n = 20/treatment; there are three treatments). Birds will be offered a control (alfalfa pellets) or sericea lespedeza pellets (75% sericea lesepedeza leaf meal pellet described above) at hatch or at 21 days of age to examine the effectiveness of sericea lespedeza pellets on preventing or controlling A. galli. Birds will be euthanized and necropsied at day 40. The number of parasites in the gastrointestinal tract will be quantified and compared between groups. Additionally, cloacal samples will be collected every 7 days for bacterial enumeration using 16S rRNA gene sequencing methods. Cloacal samples will be collected at 14 days of age and then again at the termination of the trials to determine the effect of sericea lespedeza pellets on campylobacter and salmonella.
3. Progress Report:
The project, funded through the Small Business Innovation Research Program, NIFA, USDA, is focused on evaluating a pelleted sericea lespedeza supplement for control of internal parasites on small farms. Research was conducted to determine the palatability of sericea lespedeza to poultry. Sericea lespedeza is a common perennial legume found in pastures across the southern USA that has been shown to be effective at controlling parasitic nematodes in small ruminants due to its condensed tannins content. A manuscript was published in the Journal of Applied Poultry Research. Studies were conducted to evaluate efficacy of sericia lespedeza pellets against three Emeria species in poultry. Lesion scores were reduced in birds challenged with E. tenella but not E. acervulina or E. maxima. Follow-up studies will focus on concentrating the deliverable tannins. A manuscript is under preparation.