Location: Animal Parasitic Diseases Laboratory
Project Number: 8042-32000-098-10-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Jun 1, 2011
End Date: Jun 30, 2015
The Cooperator and the assembled team of researchers has the expertise and infrastructure to 1) perform new porcine respiratory and the reproductive syndrome virus (PRRSV) infection trials in nursery pigs and pregnant gilts and porcine circovirus (PCV2) infection trials in nursery pigs; 2) perform gene expression Transcriptome Sequencing (RNA-seq) analyses of relevant samples; 3) perform single nucleotide polymorphism (SNP) analyses on genomic DNA from samples; 4) use bioinformatic resources to process genomic data for these analyses; and 5) collaborate on proteomic fluorescent microsphere immunoassays (FMIAs). An industry component for targeted gilt monitoring, sampling and application of results is included in the application.
ARS has led the PRRS Host Genetics Consortium (PHGC) Project the goal of which is to identify genetic determinants of resistance/susceptibility of commercial swine to PRRSV infection. Many of these studies have been performed with pigs from Canadian sources. The information generated in this Genome Canada proposal will extend the deep phenotyping of existing PHGC samples, will add samples from nursery infection trials with a 2nd PRRSV isolate, and will add a gilt PRRS model. These data will be used by both parties and members of the PHGC to determine genetic alleles and gene and protein expression differences that are associated with PRRS and PCV2 resistance/susceptibility. The Cooperator will perform new PRRSV and PCV2 infection trials, genotype new sets of genomic DNAs and perform RNA-seq and FMIA analyses to identify genes and proteins that differentiate PRRS resistant from susceptible pigs. The Cooperator will apply for funding through the Alberta Livestock Genomics Program grant program.