Project Number: 3625-32000-102-02-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Jun 1, 2011
End Date: May 31, 2013
To determine gene expression changes in monocytes/neutrophils isolated during the progression of a mammary gland infection in dairy cattle.
Lactating dairy cows will be infected with S. uberis. At various time points cells will be isolated from milk and sorted by cell type. Monocytes and neutrophils will be isolated and stored for subsequent RNA analysis. RNA from cells will be isolated and used to perform large-scale RNA sequencing. This data would be analyzed using current bioinformatics software designed by our collaborator. This data will become a part of a public database modeling bovine innate immunity during infections.