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United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Identify Genes and Gene Families Required for Tick Colonization

Location: Animal Disease Research

2012 Annual Report


1a. Objectives (from AD-416):
The objective of this research is to identify bacterial genes required for tick colonization.


1b. Approach (from AD-416):
A negative-selection screen will be done using a fully characterized, Francisella novicida, two-allele mutant library.


3. Progress Report:
This work relates to objective 1 of parent project by provision of pathogen genome data concerning genes required for efficient transmission. The long-term goal of this project is to identify genes that are required for tick transmission by doing negative selection screens using a mutant library from the tick transmitted bacteria, Francisella novicida. Approximately 20% of the library has been tested in a primary screen. Mutants that were negatively selected during the primary screen have deletions in genes involved in virulence, nutrient acquisition, motility, and DNA repair and recombination. A secondary screen is ongoing to verify the result from the primary screen. Information is exchanged relative to this work by regular face to face meetings and email.


4. Accomplishments

Last Modified: 2/23/2016
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