1a. Objectives (from AD-416):
Conduct a two year multi-location association mapping study to develop marker-trait associations to facilitate the development of new oat varieties with superior agronomics and disease resistance.
1b. Approach (from AD-416):
Single row plots for all 685 association lines will be grown in randomized complete block design with two replications. Agronomic traits evaluated prior to harvest will include; 1) heading date, 2) plant height, 3) lodging, and 4) maturity. Disease severity data will be collected at the appropriate time. Four hundred grams of seed will be harvested and sent to Aberdeen, ID for quality analysis including: 1) groat percentage, 2) percentage plump kernels, 3) 1000 kernel weight, 4) groat plumpness, 5) groat color, and 6) percentage broken groats using standardized methods. All data will be collected and analyzed for marker trait associations.
3. Progress Report:
This is the final report for the project. During the life of the project a total of 685 oat lines were evaluated for heading date, plant height, lodging, and maturity. The experiment was repeated over two years. Data was collected for each of the lines and sent to the CORE database for mapping analysis. In addition, 400 grams of seeds from each line was collected and sent to Aberdeen for milling quality analysis. After analysis, this data may enable plant breeders to more efficiently screen for milling quality in oat. Progress was made on the objective, which fall under National Program 301, Component 2, Crop Informatics, Genomics, and Genetic Analyses. Progress on this project focuses on problem of assessment of agronomic and rust resistance traits of oat lines. A Master’s degree candidate student in the Plant Breeding program at Texas A&M University is working on this USDA-AFRI and NAMA project entitled “Oat SNP Development and Identification of Loci Affecting Key Traits in North American Oat Germplasm Using Association Genetics”. Her thesis is entitled “Association Mapping of Crown Rust Resistance in Oat”. The student has participated in field trials to perform phenotyping of crown rust infection in 702 lines of oat comprising of spring, winter as well as core panel lines at Castroville, Texas, and Baton Rouge, Lousiana. She has also participated in some of the initial genotyping of crown rust at the USDA-ARS genotyping lab in Fargo, North Dakota under the supervision of the ARS scientist there. She is expected to graduate in July 2012. Results support Objective 3 in the parent project plan, development of improved oat lines.