Location: Crops Pathology and Genetics Research
Project Number: 2032-21000-017-09-T
Project Type: Trust
Start Date: Jan 1, 2011
End Date: Jun 1, 2012
Overall objective is to integrate molecular genetic approaches and conventional breeding methods to develop improved germplasm for the California rice industry.
Single nucleotide polymorphisms (SNPs) will be identified by sequencing reduce representation libraries of selected rice germplasm in conjunction with computational genomics. SNP markers identified in this manner will be used to assess genetic diversity and map traits of interest where possible. SNPs developed and validated by Japanese researchers will also be employed to determine the utility of these markers in analyzing California rice germplasm. Transcriptomes of various tissues and developmental stages of selected rice germplasm will be characterized by sequencing to identify genes of interest and potentially useful markers. In addition, rice plants undergoing various stresses (e.g. low temperature, stem rot disease) will also be characterized using this method which will facilitate identification of rice genes involved in responses to these stresses and, in the case of stem rot disease, fungal genes that are induced during infection and spread of the pathogen.