Location: Plant, Soil and Nutrition Research
Project Number: 8062-21000-037-04-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Nov 1, 2010
End Date: Sep 29, 2014
To identify gene expression patterns specific to individual disorders which can be developed into biomarkers for predicting apple fruit disorders, thus determining storage plans for fruit held in controlled atmosphere storage.
Facilitate RNAseq transcriptome analysis of apple tissues under conditions conducive to apple fruit postharvest disorders. We will harvest apple tissues, extract mRNA, create primer-tagged cDNA libraries, sequence said libraries via Illumina HighSeq, sort and trim sequences and process for quality, map to the public apple genome sequence and determine expression patterns via read counting. Resulting data will then feed into the larger NIFA funded apple consortium project for selection and development of biomarkers.