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United States Department of Agriculture

Agricultural Research Service

Research Project: Identification of EST-SSR and SNP Markers for Linkage Mapping in Dioscorea alata L.(Yam)

Location: Genomics and Bioinformatics Research

2012 Annual Report

1a. Objectives (from AD-416):
Preliminary Quantitative Trait Loci (QTL) mapping studies associated with anthracnose resistance and tuber quality in yam while generating SNPs for yam.

1b. Approach (from AD-416):
RNA - seq analysis of complementary Deoxyribonucleic Acid (cDNA) from resistant, susceptible genotypes and First filial generations (F1) using Illumina technology, genotyping and validation.

3. Progress Report:
The goal of this project is to develop genomics resources for improving yam breeding. Exploratory research is being conducted with the International Institute of Tropical Agriculture (IITA) as part of an agreement. Yam is an important crop in Africa but has limited genomic resources. Bioinformatic analysis was conducted on Expressed Sequence Tag (ESTs) from yam for Simple Sequence Repeats (SSRs) with the goal to identify those in the non-coding region in the hope they will be more variable than those in coding regions. A sub-set was tested on mapping parents provided by IITA. Validated DNA markers were processed on a yam mapping population related to anthracnose resistance. Work exchange takes place by email and phone conversations.

4. Accomplishments

Last Modified: 07/25/2017
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