Location: Biological Control of Pests Research2013 Annual Report
1a. Objectives (from AD-416):
The purpose of this reimbursable cooperative agreement is to analyze corn samples for mycotoxin especially aflatoxin formulation trials for two strains of non-toxigenic Aspergillus flavus (A. flavus), K 49 & NRRL 21882.
1b. Approach (from AD-416):
Several Corn Samples will be delivered by Syngenta and tested in an ARS laboratory using HPLC or LC/MS method. The following procedures and tasks will be carried out by USDA and Syngenta personnel: Syngenta will deliver to Investigator one combined sample per plot. ARS Investigator will grind, subsample and determine Aflatoxin levels for four major strains.
3. Progress Report:
Field corn samples (total 310 samples) were received in fall of 2012 and analyzed for Aflatoxins, fumonisin, and Cyclopiazonic Acid (CPA) by High Performance Liquid Chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA) and Liquid Chromatography/ Mass Spectrometry (LC/MS). Fungal isolation of Aspergillus flavus strains were isolated from several samples. All data and results were reported to the cooperator. Monitoring of this project continues through frequent visits, telephone and e-mail communications.