1a. Objectives (from AD-416):
The purpose of this project is to develop and deploy effective stripe rust resistance genes in the next generation of U.S. barley varieties, including germplasm from the ARS-Aberdeen breeding program. In this project we have developed the following objectives: 1. Use current barley stripe rust resistance QTL alleles via marker assisted selection (MAS). a. We will transfer BISON resistance alleles to winter malting 2-rows, winter food 2-rows and winter food 6-rows and OR71 resistance alleles to Nebraska cold tolerant germplasm. 2. Discover new resistance QTLs/genes. a. We will leverage T-CAP association mapping (AM) results for barley stripe rust (BSR) resistance. 3. Confirm, validate, and identify resistant germplasm in addition to OSU program. a. We will expand our screening of USDA-ARS germplasm for resistance at Corvallis.
1b. Approach (from AD-416):
Use current alleles via MAS: We will leverage genotyping services provided by the Triticeae-CAP (T-CAP) project at the USDA Regional Lab, Pullman. In collaboration with Deven See at this facility, we have developed Sequenom assays to characterize stripe rust resistance haplotypes in the following germplasm: (i) doubled haploid (DH) lines derived from crosses of European winter 2 –rows and the BISON, (ii) F4 head rows derived from crosses of BISON resistance with hull-less food types, and (iii) BC1 progeny derived from crosses with Nebraska cold tolerant germplasm. The resistance haplotypes will be validated using field resistance data and used for marker assisted selection (MAS). Discover new resistance QTLs/genes: We will leverage T-CAP Association Mapping (AM) for BSR resistance in the national collection. This germplasm resource will be phenotyped and genotyped in 2012 via T-CAP. Based on the AM, with support from this SCA and in collaboration with personnel at Aberdeen, in the Fall 2012 we will initiate crossing to introgress new QTL alleles/genes into adapted winter germplasm. Confirm, validate, and identify resistant germplasm in addition to OSU program: Because barley stripe rust is not endemic at Aberdeen, Idaho, we will expand our screening of USDA-ARS germplasm for resistance at Corvallis. This will be accomplished by fall-planting in 2011 to ensure optimum epidemic development in spring, 2012. Dissemination and publication: All germplasm will be publicly available: We will prepare and/or co-author peer reviewed publications with USDA-ARS scientists at Aberdeen, Idaho.
3. Progress Report:
Gene discovery, plant breeding, and variety release should be integrated endeavors. When they are integrated, farmers have the direct benefit of superior varieties, processors have the benefit of an assured source of supply, and consumers enjoy an uninterrupted flow of healthy plant-derived products. At the other end of the pipeline, the scientific community benefits from an expanded body of knowledge. In the case of barley stripe rust, we have systematically mapped genes conferring durable resistance, we have transferred these genes to adapted germplasm, and we have released the winter barley “Alba”. Alba was approved as a public release by the Oregon Agricultural Experiment Station in 2012. More information on this variety is available at http://barleyworld.org/breeding-genetics/food-feed-forage and a variety release document will be submitted to the Journal of Plant Registrations. Alba has shown excellent resistance to barley stripe rust, and another important disease of barley – scald – over years and locations. Although intended as a feed variety, Alba can be pearled to provide an attractive, nutritious, and healthy food and it has also been used for production of specialty craft beers. Results of this project relate to Sub-objective 2B of the parent project, “Introgress quantitative and qualitative resistance to barley stripe rust into adapted germplasm via marker assisted selection (MAS)".