1a. Objectives (from AD-416):
The overall goal of the 'T-CAP' project is to phenotype and genotype diverse and designed germplasm pools to mine and deploy alleles that improve barley and wheat adaptation and performance under biotic and abiotic stress, and couple these activities with the training of a new generation of plant breeders. Specific objectives for the work at Aberdeen, ID are 1) Evaluate NSGC accessions from the core collection for nitrogen use efficiency (NUE) and water-use efficiency (WUE)in cooperation with University of Idaho colleagues. 2) Evaluate barley lines in an association mapping panel for yield, NUE, and WUE in the field at Aberdeen.
1b. Approach (from AD-416):
Within the overall T-CAP project, three teams of experienced barley and wheat breeders will provide standardized field-based evaluations of WUE, NUE, and yield. Physiologists will develop high through put phenotyping methods for drought, heat and NUE. The generated datasets will be used to identify valuable alleles for these traits and to deploy them using MAS and GS approaches. The project will use available Illumina SNP platforms to genotype the complete barley (2,571 accessions) and wheat (5,490 accessions) core NSGC collections. We will also phenotype the complete core collections for stem, leaf and stripe rust and other important pathogens. A subset of 2,000 accessions will be screened in year one for abiotic stresses using 20 nurseries grouped by height and heading time. Promising lines (600) will be characterized in more detailed experiments in years 2 to 5. Based on the phenotypic and genotypic data, subsets capturing the genetic diversity in the NSGC collection will be crossed with elite varieties to generate nested association mapping (NAM) and other segregating populations for evaluation in years 4 and 5.
3. Progress Report:
This project relates to the parent project’s Objective 2: “Conduct genetic characterizations and phenotypic evaluations of wheat, barley, oats, rye, and other small grains and related wild species for priority genetic and agronomic traits.” This work is part of an effort to find markers associated with nitrogen and water use efficiency that can eventually be used to breed more efficient barley cultivars. Our location grew the spring, two-row elite breeding association mapping panel, which consisted of barley lines gathered from barley breeders in North America. There were 256 non-replicated lines placed in an augmented block, along with 44 check plots (Harrington 21 times, Baronesse, Hockett, and MT090183-6 times, Pinnacle-5 times) planted in each of the five blocks. The experimental lines and checks were randomly distributed among the five blocks for a total of 300 lines. Three treatments were used for each of the 300 lines: Normal water and nitrogen, reduced water (36% of normal water) and normal nitrogen, and reduced nitrogen (72% of normal nitrogen) and normal water. Agronomic data collection included heading date (Julian), lodging percent of plot, plant height, and grain yield. Grain quality data collection included, plump grain, grain protein, and test weight. Morphological data collection included measurement of stem length. Soil samples were taken from each of the check plots shortly after planting and again at harvest and sent for nutrient profile analysis. Other field management activities including, irrigation, herbicide application and fertilization were recorded. Canopy spectral reflectance measurements were taken at three different intervals spaced approximately one week apart after heading and before harvest. All agronomic data for the 2012 season has been uploaded to the Triticeae toolbox web site, with the canopy spectral reflectance data to follow. The 2013 trial has been planted and field observations are underway.