1a. Objectives (from AD-416):
The overall goal of the 'T-CAP' project is to phenotype and genotype diverse and designed germplasm pools to mine and deploy alleles that improve barley and wheat adaptation and performance under biotic and abiotic stress,and couple these activities with the training of a new generation of plant breeders. Specific objectives for the work at Aberdeen, ID are 1) Evaluate NSGC accessions from the core collection for nitrogen use efficiency (NUE) and water-use efficiency (WUE)in cooperation with University of Idaho colleagues. 2) Evaluate barley lines in an association mapping panel for yield, NUE, and WUE in the field at Aberdeen.
1b. Approach (from AD-416):
Within the overall T-CAP project, three teams of experienced barley and wheat breeders will provide standardized field-based evaluations of WUE, NUE, and yield. Physiologists will develop high throughput phenotyping methods for drought, heat and NUE. The generated datasets will be used to identify valuable alleles for these traits and to deploy them using MAS and GS approaches. The project will use available Illumina SNP platforms to genotype the complete barley (2,571 accessions) and wheat (5,490 accessions) core NSGC collections. We will also phenotype the complete core collections for stem, leaf and stripe rust and other important pathogens. A subset of 2,000 accessions will be screened in year one for abiotic stresses using 20 nurseries grouped by height and heading time. Promising lines (600) will be characterized in more detailed experiments in years 2 to 5. Based on the phenotypic and genotypic data, subsets capturing the genetic diversity in the NSGC collection will be crossed with elite varieties to generate nested association mapping (NAM) and other segregating populations for evaluation in years 4 and 5.
3. Progress Report:
The National Small Grains Collection has a wealth of diverse small grains accessions. To help better understand the genetic diversity within the collection, we are analyzing the SNP marker data for the barley and wheat ‘core’ subsets. The marker data was generated as part of the T-CAP project. After the analysis is complete, we will identify ‘mini-cores’ for both barley and wheat that will capture much of the diversity within the core subsets but will have many fewer accessions. The mini-cores will be useful for evaluating traits that are expensive to assay—such as certain grain quality attributes—and help us to better target future evaluations of the collection materials. The results relate to Objective 2 in the parent project, “Strategically evaluate small grains genetic resources for priority biotic and abiotic stress resistance.”