Project Number: 5438-41630-001-05-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2010
End Date: Jan 14, 2013
Develop and optimize the effectiveness of bioremediation techniques for the abatement of odorous compounds in CAFO wastewater. Optimize the effectiveness of genetically modified yeast for abatement of odorous compounds. Evaluate a non-GMO based anaerobic bioreactor with complementary attributes.
A continuous flow bioreactor will be fabricated, optimized, and tested based upon genetically modified Saccharomyces cerevisiae (Baker’s yeast) displaying horseradish peroxidase (HRP). HPLC methods will be used for liquid phase quantification of phenolic and indolic odorants, and for CAFO manure lagoon and bioreactor performance optimization and evaluation. Effectiveness of the odor control and VOC remediation will be measured using GC/MS and thermal desorption tubes.