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United States Department of Agriculture

Agricultural Research Service

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Research Project: Novel Real-Time PCR-Based Surveillance Systems for Candidatus Liberibacter Species, Bacteria Associated with Citrus Huanglongbing

Location: Crop Diseases, Pests and Genetics Research

Project Number: 5302-22000-010-06-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Dec 1, 2010
End Date: Nov 30, 2012

1. To develop ultra sensitive Taq-Man PCR diagnostic system for improved detection of Ca. Liberibacter asiaticus. 2. To design a Ca. Liberibacter genus-specific real-time PCR technology for general detection of Candidatus Liberibacter species.

1. Cloning, sequencing and characterizing genomic sequences of citrus Candidatus Liberibacter species using novel molecular technique. 2. Design and validate sensitivity and specificity of diagnostic systems.

Last Modified: 10/15/2017
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