1a. Objectives (from AD-416):
To design and optimize semiochemical-based methods for control of major insect pests such as the citrus leafminer, Phyllocnistis citrella. In addition to field trials, laboratory research will address fundamental aspects of mechanisms underlying mating disruption.
1b. Approach (from AD-416):
Replicated, multilocational field trials will be established in commercial citrus groves to document application rates, coverage and deployment methods to optimize control of major citrus pests such as the citrus leafminer and associated citrus canker disease. Signals from antennae of citrus leafminer and other insects of interest will be recorded in response to odors collected from host plants and conspecifics. Antennae will be connected to a gas chromatograph-coupled electroantennogram (GC-EAD) and compounds of interest will be identified by GC-Mass Spectrometry (GC-MS).
3. Progress Report:
This project is directly related to Objective 1 of the parent project: Develop semiochemical-based control methods for citrus pests, particularly Asian citrus psy1lid (ACP). Improvements to semiochemical-based methods of control were pursued for citrus leafminer (CLM). Field trials were conducted in collaboration with the University of Florida, Lake Alfred to identify optimize control of CLM through mating disruption (MD) of CLM. Field experiments demonstrated a failure in trap catch disruption following application of CLM pheromone in a slow-release matrix, SPLAT™ (Specialized Pheromone & Lure Application Technology, ISCA Technologies Inc., Riverside, CA). Field trials were established to test the longevity of attraction using solid rubber pheromone dispensers in place of SPLAT CLM. Longevity of trap catch disruption using rubber dispensers exceeded 30 weeks compared with the best result to date of 8 weeks of disruption using SPLAT CLM. Studies of flight behavior revealed that females may be able to fly in excess of 1.2 km from infested citrus. Future studies are planned to test mating disruption in commercial groves.