Project Number: 2050-21000-032-04-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 14, 2011
End Date: May 10, 2016
Develop Potato Virus Y (PVY) resistance in existing cultivars by: 1)testing the S. hirsutum pot-1 gene as a source of PVY resistance in potato; 2) doing a molecular characterization of eIF4E-transgenic lines; and 3) produce minitubers for proof of concept field studies.
Inoculation studies will be done using Potato Virus Y O strain (PVYO) Potato virus Y NTN strain (PVYNTN), and Potato virus Y N:O strain (PVYN:O) strains in a greenhouse to assay the resistance in four cultivars transformed with the pot-1 gene. Molecular characterization of the transgenic lines will be assayed for sequence insertion and copy number using a Southern Blot analysis. Minitubers of the transgenic lines will be produced in sufficient numbers to allow replicated field trials for PVY resistance in 2012.